Optical Constant Determination of Bacterial Spores in the MIR

Abstract

We developed a simple method for estimating the real and imaginary refractive index of particles. This is done by filtering the particles (bacterial spores in our case) on a gold-coated filter membrane and performing IR microspectroscopy. The results are extremely reproducible (within particle counting statistics). A Kramers-Kronig consist iterative algorithm is applied to obtain scattering and absorption cross-sections for individual particles. The method was tested on spores and on polystyrene spheres.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Dec 05, 2005
Accession Number
ADA440955

Entities

People

  • Michael L. Myrick

Organizations

  • University of South Carolina

Tags

Communities of Interest

  • Human Systems

DTIC Thesaurus Topics

  • Absorption
  • Absorption Cross Sections
  • Chemistry
  • Dielectric Polymers
  • Filters
  • Filtration
  • Membranes
  • Microscopes
  • Microscopy
  • Optical Properties
  • Optics
  • Particles
  • Physical Properties
  • Refractive Index
  • Remote Sensing
  • Scattering
  • Spores

Fields of Study

  • Physics

Readers

  • Aerosol Science/Aerosol Physics
  • Approximation Theory.
  • Spectroscopy.