Optical Spectroscopy and Multiphoton Imaging for the Diagnosis and Characterization of Hyperplasias in the Mouse Mammary Gland
Abstract
There is no reliable method for identifying pre- or non-malignant lesions in animal models of breast cancer in vivo and thus no method for studying carcinogenesis in vivo. The purpose of this project is to develop a method to diagnose mammary gland hyperplasias in an animal model in vivo using optical spectroscopy. The absorption and scattering parameters extracted from diffuse reflectance spectra measured in vivo in the 400-600 nm range were used to differentiate normal tissue (n=23) and benign lesions (n=16) of the exposed mammary glands of 17 ENU-treated FVBxB6 Apc (Min/+) mice. Malignant lesions (n=3) were identified by palpation. Wilcoxon rank sum tests revealed a statistically significant increase (p<0.05) in total hemoglobin concentration for benign lesions compared to the normal mammary gland. Statistically significant differences (p<0.05) in the mean reduced scattering coefficient were also found between two sub-classes of benign lesions. Subsequent studies will focus on the differentiation of normal and malignant lesions. In the future, this technique could be used to noninvasively determine which benign lesions ultimately become malignant. This has important implications for longitudinal disease progression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2005
- Accession Number
- ADA441356
Entities
People
- Melissa C. Skala
Organizations
- University of Wisconsin–Madison