ENU Mutagenic Screen for Susceptibility and Resistance to Streptococcus Pneumoniae
Abstract
The main goal of the DARPA HOT program is to determine the ability of the host organism to provide protection against bacterial and viral infection. The genetic pathways that control susceptibility and resistance to bacterial infection have remained poorly understood, because of the lack of expertise in the development of techniques capable of identifying factors that are involved in the process. The proposal outlined here will use chemical mutagenesis with N-ethyl-N-nitrosourea (ENU) combined with the resources of mouse genomics to identify genes that are involved in the susceptibility and resistance to bacterial infection. Male Balb/c mice (G0) will be subjected to ENU mutagenesis using standard protocols and crossed to female C57BL/6 mice to generate G1 offspring. The G1 generation will be subjected to a lethal dose (LD-100) of streptococcus pneumonia and animals that are resistant and highly susceptible will be identified. The G0 males of the resistant G1 offspring will be outcrossed onto the C57BL/6 background to facilitate the genetic mapping of the mutations by haplotype analysis. The animals will be analyzed to identify the chromosomal locus and eventual identification of the gene(s) responsible for susceptibility and resistance to bacterial infection.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 07, 2005
- Accession Number
- ADA441504
Entities
People
- Mark S. Kindy