Methods of Analysis by the U.S. Geological Survey Organic Geochemistry Research Group - Determination of Glyphosate, Aminomethylphosphonic Acid, and Glufosinate in Water Using Online Solid-Phase Extraction and High-Performance Liquid Chromatography/Mass Spectrometry
Abstract
An analytical method for the determination of glyphosate, its principal degradation compound, aminomethylphosphonic acid (AMPA), and glufosinate in water with varying matrices has been developed. Four different sample matrices forti ed at 0.2 and 2.0 g/L (micrograms per liter) were analyzed using precolumn derivatization with 9- uorenylmethylchloroformate (FMOC). After derivatization, cleanup and concentration were accomplished using automated online solidphase extraction followed by elution with the mobile phase allowing for direct injection into a liquid chromatograph/mass spectrometer (LC/MS). Analytical conditions for MS detection were optimized, and quantitation was carried out using the following representative ions: 390 and 168 for glyphosate; 332, 110, and 136 for AMPA; and 402, 180, and 206 for glufosinate.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2002
- Accession Number
- ADA441991
Entities
People
- A. P. Strahan
- E. A. Lee
- E. Michael Thurman
Organizations
- United States Department of the Interior