Function of Human Selenium-Binding Protein in Selenium Induced Growth Arrest and Apoptosis in Prostate Cancer Cells
Abstract
The aim of the study is to elucidate the role of human selenium binding protein (hSP56) in selenium induced cell apoptosis in cancer cells. During the second year of the project we demonstrated that the human prostate (PC-3) cells overexpressing hSP56 protein show changed growth characteristic and increased sensitivity to selenium compounds. To understand the biological role(s) of hSP56, a yeast two-hybrid screening has been performed to identify\ the proteins interacting with hSP56 from human prostate cDNA library. The screening identified two potential proteins as interacting partners of hSP56, Ubc12 and VDU1. Both proteins are involved in ubiquitination-mediated protein degradation pathway. We hypothesize that hSP56 regulates the protein degradation pathway by altering the properties of Ubc12 and/or VDU1.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2005
- Accession Number
- ADA442188
Entities
People
- Natasa Debeljak
Organizations
- Beth Israel Deaconess Medical Center