Gas Chromatographic-Mass Spectrometric Determination of British Anti-Lewisite in Plasma
Abstract
British anti-Lewisite (BAL) (2,3-dimercapto-1-propanol) is a potential therapeutic compound when used against the effects of cutaneous sulfur mustard, and a method for its determination in plasma has been developed. BAL and the internal standard (IS) ethane dithiol were isolated from plasma samples through solid-phase extraction and then reacted with 1-pentafluoropropionylimidazole, forming stable pentafluoropropionyl derivates that are sensitive to gas chromatographic-mass spectrometric analysis. Examination of concentration versus peak-area ratios of the BAL and IS derivatives demonstrated the method to be linear over a concentration range of 0.48 to 124 ng(mL in plasma when fit to a weighted (1/y2) least squares regression. Correlation coefficients were 0.9943 to 0.9995 for six runs, and coefficients of variation (CV) were 2.5 to 8.7% over the eight concentrations tested. The intra- and interday accuracy and precision of this method was measured by examining six groups of eight unknown test samples (n = 6).
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2004
- Accession Number
- ADA442484
Entities
People
- B. R. Capacio
- C. E. Byers
- E. D. Clarkson
- E. R. Holloway
- G. E. Platoff
- Joseph P. Smith
- W. D. Korte
Organizations
- United States Army Medical Research Institute of Chemical Defense