Normal Human Astrocyte Instructions for Initiation of Cultures from Cryopreserved Cells and Subculture

Abstract

This technical report outlines procedures that have been developed to provide a cost-effective way to produce large quantities of Normal Human Astrocyte (NHA) cells for studying the mechanism(s) of action of chemical warfare agents (CWAs) and medical countermeasures against CWAs. Five milliliters of a supplement provided by Clone Express was added to 467 mL of DMEM F12 media as well as 25 mL of fetal bovine serum plus 0.3 mL gentamicin and 2.5 mL of fungizone. Cell viability and cell proliferation were evaluated using the Coulter Z1 Particle Counter.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2004
Accession Number
ADA442897

Entities

People

  • Carmen M. Arroyo
  • David W. Kahler

Organizations

  • United States Army Medical Research Institute of Chemical Defense

Tags

DTIC Thesaurus Topics

  • Astrocytes
  • Biological Sciences
  • Biomedical Research
  • Cell Line
  • Cells
  • Chemical Warfare
  • Chemical Warfare Agents
  • Chemistry
  • Counters
  • Culture Techniques
  • Environmental Pollutants
  • Medical Personnel
  • Nerve Agents
  • Particle Counters
  • Particles
  • Peptide Growth Factors
  • Shelf Life

Readers

  • Critical Infrastructure Protection in CBRN and WMD Threats.
  • Immunology