Proteomic Analysis of Genistein Mammary Cancer
Abstract
Using 2-D gels and mass spectrometry we have discovered that GTP-cyclohyrolase I protein expression is significantly increased in mammary glands of 21 day old rats, but not in 50 day old rats following prepubertal only exposure to the phytoestrogen genistein. By evaluating related metabolic pathways we found up-regulated P-ERK-I, but no significant short-term changes in the tyrosine hydroxylase and INOS protein expressions in mammary glands of 21 day old rats. At day 50, there was significant up-regulation of tyrosine hydroxylase and VEGF-R2. This and previous work suggests that early postnatal (prepubertal) exposure to genistein enhances cell proliferation and cell differentiation and gland maturation. This unique development maturation leads to a new biochemical "blue-print" whereby the cells have reduced EGF-signaling and VEGF-R2 that render the mature mammary gland less proliferative and susceptible to chemically induced mammary cancer initiation, angiogenesis and for cancer progression. This study demonstrates the usefulness of proteomics for the discovery of novel pathways that may be involved in cancer prevention. Our ongoing work is to collect proteins from interstitial fluid surrounding mammary glands of rats, and to identify and characterize the major proteins that are modulated by DMBA.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2005
- Accession Number
- ADA442989
Entities
People
- Coral A. Lamartiniere
Organizations
- University of Alabama