Operative Therapy and the Growth of Breast Cancer Micrometastases: Cause and Effect

Abstract

To test the hypothesis that there are growth factors/cytokines released to promote healing of the wound created by the resection of a primary breast cancer which have the unintended consecuence of enabling the growth of micrometastatic foci present at the time of operation. This study was designed as a feasibility study to test whether it is technically possible to reliably assay changes in the low molecular weight serum proteome. Human xenograft breast tumors were established in 9 of 12 nude mice. Blood samples were obtained from the mice immediately prior to extirpation of the primary breast cancer and then again 24 hours, 48 hours and 7 day post-operatively. In the analysis of the serum of 5 of the mice there were 8685 peptides quantified resulting in 5949 proteins. Of these 5949 proteins 155 were identified with high confidence and 11 proteins had significant changes among the time points as a function of time pre or post-op.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2005
Accession Number
ADA443039

Entities

People

  • Susan E. Clare

Organizations

  • Indiana University

Tags

DTIC Thesaurus Topics

  • Blood Proteins
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Cytokines
  • Databases
  • Growth Factors
  • Health Services
  • Information Science
  • Liquid Chromatography
  • Neoplasms
  • Peptide Growth Factors
  • Peptides
  • Proteins
  • Proteomics
  • Stem Cells

Readers

  • Oncology (Cancer Research).
  • Oncology and Biomarker-Based Cancer Detection.
  • Trauma Surgery or Emergency Medicine.

Technology Areas

  • Biotechnology