Apoptosis and Tumor Progressionin Prostate Cancer
Abstract
In the period covered by this application we have repeated a large scale animal study to investigate the effect of chronic exposure of PC-346C(RFP) (which expresses the wild type receptor) to Casodex. The intermediate criteria we are monitoring are primary tumor volume, rates of proliferation (as measured by BrdU incorporation) and apoptosis as measured by TUNEL staining. We have standardized an efficient methodologies for isolating cells from primary tumors expressing REP by fluorescence activated cell sorting (FACS) and by laser capture micro-dissection (LCM) . We have intitiated a comprehensive micro-array based bioinformatics effort to identify genes whose expression is modulated by Casodex to characterize the molecular events underlying metastatic progression of PC-346C(RFP) cell. The changes in gene expression detected by micro-array are being validated by QT-PCR using SYBR green. The results of these experiments are also being compared to the effects of Casodex in LNCaP cells (which has a mutated androgen receptor), and to the changes in gene expression seen in the PC-346C(RFP) primary tumors (and metastases) from animals chronically treated with Casodex. These studies will identify changes in cell behavior and gene expression that lead to the acquisition of an invasive phenotype.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2005
- Accession Number
- ADA443063
Entities
People
- Martin P. Tenniswood
Organizations
- University of Notre Dame