Analysis of Genes Differentially Expressed in a Human Ovarian Cancer Model

Abstract

The objectives of this study were to (1) analyze gene expression in a model of human ovarian cancer from a benign to a malignant phenotype in cells grown in organotypic culture and (20 confirm gene expression patterns by RNA analysis. Two human cell lines were used, 96.9.18 and 1.24.96, and RNA extracted from cells at various stages of malignant change were queried on Affymetrix HU133A and B chips in triplicate (96.9.18) or HU133A plus chip in triplicate (1.24.96) . Data analysis has revealed a number of genes that are dysregulated in both 96.9.18 and 1.24.96. An analysis of the interaction of these genes showed that 5 genes are downregulated between the two cell lines and 183 genes are upregulated during malignant progression. Downregulated genes are too few to analyze but the upregulated genes are involved in apotosis, cytokine-cytokine receptor interactions, toll-like receptor signaling pathway, and the MAPK signaling pathway.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2005
Accession Number
ADA443203

Entities

People

  • Wayne D. Lancaster

Organizations

  • Wayne State University

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Cancer
  • Cell Line
  • Cells
  • Culture Techniques
  • Cytokines
  • Data Analysis
  • Data Science
  • Dna Microarrays
  • Epithelial Cells
  • Gene Expression
  • Genetics
  • Genome
  • Neoplasms
  • Ovarian Cancer
  • Phenotypes
  • Polymerase Chain Reaction

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular and Cellular Biology