Functional Study of the Human BRCA2 Tumor Suppressor

Abstract

My research is focused on the BRCA2 protein, whose mutations have been implicated in the development of breast, ovarian, male breast, prostate, pancreatic cancers and Fanconi anemia. It is intended to elucidate some of the biological functions of BRCA2 and/or regulation of its in vivo function through generation/utilization of new reagents and identification of new BRCA2 interacting proteins. During the years of grant support, I generated an array of BRCA2 antibodies and identified/cloned a novel protein, PALB2, as a major nuclear partner and localizer of BRCA2. I have established that PALB2 is required for BRCA2 nuclear presence and its functions in homologous recombinational repair of double-strand breaks and intra-S phase damage checkpoint control. Importantly, BRCA2/PALB2 interaction is disrupted by multiple cancer-associated BRCA2 mutations. My results indicate that PALB2 is essential for the tumor suppressing functions of BRCA2 and may be a new tumor suppressor gene in its own right.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2005
Accession Number
ADA443610

Entities

People

  • Bing Xia
  • David M. Livingston

Organizations

  • Dana–Farber Cancer Institute

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Amino Acids
  • Androgen Receptors
  • Antibodies
  • Biomedical Research
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Cytoplasm
  • Epithelial Cells
  • Intranuclear Space
  • Ionizing Radiation
  • Neoplasms
  • Proteins
  • Radiation
  • Suppressors
  • Tissue Extracts

Readers

  • Molecular Biology and Genetics
  • Molecular and genetic basis of cancer.