Development of a Manual Threshold Immunoassay for Bacillus anthracis Spores
Abstract
An immunoassay applicable to the Manual Threshold instrument was developed for the detection of Bacillus anthracis spores. The assay involved two antibodies raised against inactivated spores of B. anthracis Sterne strain 34F2, and they were labelled with biotin and fluorescein for capture and detection, respectively. The assay was linear over the range of 0 - 4x10,000 spores/ml. The limit of detection was estimated to be approximately 500 spores/ml. The detection response for B. anthracis spores was at least 10-fold more sensitive than for the spores of Bacillus cereus, Bacillus thuringiensis and Bacillus globigii. The assay was also used to detect B. anthracis spores from aerosol samples.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2005
- Accession Number
- ADA443697
Entities
People
- Chun-qiang Liu
- Cindy Browning
Organizations
- Defence Science and Technology Group