Interrogating Androgen Receptor Mediated Gene Expression and Tumor Progression by Molecular Imaging

Abstract

This final report summarizes our work on the effectiveness of a new adenovirus based optical imaging vector designed to detect MAP kinase levels in living subjects. The vector was injected into human tumors implanted into SCID mice. Animals were injected with D-luciferin and imaged in a Xenogen IVIS device. The vector was able to detect enhanced MAPK levels stimulated by Epidermal Growth Factor in prostate cancer tumors of xenograft animals. We interrogated two lines of androgen dependent (AD) and independent (Al) xenograft animal models (CWR22 and LAPC9) to determine MAPK levels. We found no significant difference between AD and Al cancer with respect to functional MAPK levels. However, CWR22 animals exhibit significantly higher MAPK levels than LAPC9 xenograft animals. The data suggest the possibility that gene expression based imaging may be able to detect augmented MAPK levels predictive of disease progression as well as the efficacy of cancer therapeutics.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2005
Accession Number
ADA443777

Entities

People

  • Michael F. Carey

Organizations

  • University of California, Los Angeles

Tags

DTIC Thesaurus Topics

  • Androgen Receptors
  • Biological Sciences
  • Cell Physiological Processes
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Department Of Defense
  • Gene Expression
  • Growth Factors
  • Health Services
  • Medical Personnel
  • Molecules
  • Neoplasms
  • Prostate Cancer
  • Proteins
  • Small Molecules
  • Therapy

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Medical Imaging.
  • Prostate Cancer Biology.