Dendritic Cell-Based Immunotherapy of Breast Cancer: Modulation by CpG DNA
Abstract
Breast cancer is the most common non-skin cancer in women and the American Cancer Society estimates that there will be 215,990 new cases of invasive breast cancer and 40,110 deaths from MBC in the United States in 2004. Thus, patients with MBC who fail conventional therapies are candidates for clinical trials using novel therapeutic approaches, including immunotherapy. Dendritic cells (DC) are potent antigen-presenting cells that prime antitumor cytotoxic T lymphocytes against tumor-associated antigens and bacterial DNA oligodeoxynucleotides containing unmethylated CpG sequences (CpG DNA) further augment the immune priming functions of DCs. We hypothesize that CpG DNA-stimulated DCs will prime a more potent anti-tumor immune response than non-stimulated DCs. Our 3 specific aims are 1) to study the mechanism of antitumor immunity mediated by the vaccination of TS/A mammary tumor-bearing BALBIc mice with CpG DNA-stimulated DCs primed in vitro with necrotic TS/A cells, 2) to determine optimal conditions for CpG DNA stimulation and tumor priming of human DCs, and 3) to design a Phase I/II clinical trial of DC + CpG DNA therapy for breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2005
- Accession Number
- ADA444026
Entities
People
- Joseph Baar
Organizations
- University of Pittsburgh