RAR Beta: Actions in Prostate Cancer

Abstract

I have previously identified some RARbeta% targets genes. I found expression of one of the target gene, ELF3, is reduced in prostate cancer cells as compared to normal prostate epithelial cells. In the past year, I carried out transient transfection study to delineate how RA and RARBETA beta transcriptionally regulate ELF3 gene expression. I cloned a 1-kb ELF3 promoter into a luciferase reporter construct. Transient transfection assays showed that RA induced activity of the 1-kb ELF3 promoter (ELF3-luc) about 2.3 fold in F9 wt cells. I also showed that retinol could induce the transcription of COUP-Tfs expression RARBETA and its target genes in F9' teratocarcinoma cells at lower doses than RA. COUP-Tfs are nuclear receptors that have been shown to be required for the induction of RARBETA transcription. They could play a role in the regulation of RARBETA expression in prostate cancer.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2005
Accession Number
ADA444657

Entities

People

  • Yong Zhuang

Organizations

  • Cornell University

Tags

DTIC Thesaurus Topics

  • Amines
  • Antineoplastic Agents
  • Breast Cancer
  • Cancer
  • Carcinoma
  • Cell Line
  • Cells
  • Chemistry
  • Diseases And Disorders
  • Epithelial Cells
  • Gene Expression
  • Liquid Chromatography
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Tissue Extracts
  • Tissues

Fields of Study

  • Biology

Readers

  • Applied Combinatorial Optimization and Logic Circuit Design.
  • Breast cancer cell signaling and growth regulation.