In Vitro Toxicity of Aluminum Nanoparticles in Rat Alveolar Macrophages
Abstract
The purpose of this research was to investigate and characterize the in vitro cellular effects of exposing rat lung macrophages to aluminum oxide nanoparticles (30 and 40nm average size) compared to aluminum metal nanoparticles (50, 80, and 120nm). This study used toxicity endpoints involving cell viability, mitochondrial function, phagocytotic ability, and inflammatory response. Results indicated none to minimal toxicological effects occurred with exposure of macrophages as high as 500 microg/ml for 24 hours with aluminum oxide nanoparticles. However, there was significant delayed toxicity that occurred at 96 and 144 h post exposure. Exposure to aluminum metal nanoparticles indicated slight to moderate toxicity after 24 hours exposure at 100 and 250 microg/ml. The phagocytic ability of these cells was significantly hindered by exposure to all tested aluminum nanoparticles at 25 microg/ml for 24 hours, but not by the aluminum oxide nanoparticles. A series of cytokine and nitric oxide assays performed showed aluminum nanoparticles did not induce an inflammatory response.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2001
- Accession Number
- ADA445568
Entities
People
- Andrew J Wagner
- Charles Bleckmann
- E. England
- John J. Schlager
- Krista Hess Saber /hussain
Organizations
- Air Force Research Laboratory