In Vitro Toxicity of Aluminum Nanoparticles in Rat Alveolar Macrophages
Abstract
The purpose of this research is to characterize the in vitro cellular effects of rat lung macrophages to exposure to aluminum oxide nanoparticles (Al2O3-NP) (30 and 40nm) compared to aluminum nanoparticles (Al-NP) (50, 80, and 120nm). This study concentrates on cell viability, mitochondrial function, phagocytosis ability, and cytokine response. Results indicate no to minimal toxicological effects on macrophages exposed as high as 500 μg/ml for 24 hours with Al2O3-NP. However, there was a significant delayed toxicity that occurred at 96 and 144 h post exposure. Al-NP shows sight to moderate toxicity after 24 h exposure at 100 and 250 μg/ml. The phagocytic ability of these cells was significantly hindered by exposure to each size of the Al-NP at 25 μg/ml for 24 hours, but not by the Al2O3-NP. A series of cytokine and nitric oxide assays performed show none of these aluminum nanoparticles are inducing an inflammatory response.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2006
- Accession Number
- ADA446266
Entities
People
- Andrew J Wagner
Organizations
- Air Force Institute of Technology