MMP-8: A Breast Cancer Bone Metastasis Suppressor Gene
Abstract
In order to study the role of MMP-8 on inhibition of cancer growth and progression, we initiated our work to clone the human MMP-8 cDNA and express it in vitro. The pcDNA3.1 contains the following elements: human cytomegalovirus (CMV) immediate-early promoter/enhancer that permits efficient, high-level expression of recombinant protein and V5 epitope that allows detection of recombinant protein with anti-V5 antibody. The MMP-8 cDNA with a V5-epitope tag was cloned downstream into the CMV promoter sequence. The construct pCMV-MMP-1-V5 was sequenced to verify cloning of the MMP-8 cDNA insert in frame. The molecular mechanisms of how TGF-beta1 mediates stimulation of invasion and formation of bone metastases have yet to be completely determined. In my laboratory, we have found that ATF-3 (activating transcription factor- 3) is strongly stimulated and its level is sustained by TGF-beta1 in highly invasive and bone metastatic human breast cancer cells. A defect in repression of ATF-3 expression in breast cancer cells could lead to activation of genes that participate in multi-step breast cancer progression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2005
- Accession Number
- ADA446267
Entities
People
- Nagarajan Selvamurugan
Organizations
- University of Medicine and Dentistry of New Jersey