Chemical Genetics of 14-3-3 Regulation and Role in Tumor Development

Abstract

The 14-3-3 proteins are central to cell growth and have been implicated in the regulation of tumor development. While much is known about their mode of function in that they bind to ligands and affect their cellular activity, the regulation of 14-3-3s is not clearly understood. For this we have developed a cell-based assay to screen for modulators of 14-3-3 functions. Specifically we have screened a chemical compound library, and more recently as a follow up, a kinase ORF and a kinase siRNA library for molecules that affect the transport of 14-3-3 sigma from the nucleus to the cytoplasm. We have identified a number of candidate hits that we describe here and which we are currently in the process of characterizing.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2005
Accession Number
ADA446328

Entities

People

  • Garabet G. Toby

Organizations

  • Dana–Farber Cancer Institute

Tags

DTIC Thesaurus Topics

  • Apoptosis
  • Biology
  • Biomedical Research
  • Cell Physiological Processes
  • Cells
  • Cellular Structures
  • Chemical Compounds
  • Chemical Synthesis
  • Chemistry
  • Cytoplasm
  • Genetics
  • Human Genome
  • Molecules
  • Neoplasms
  • Prostate Cancer
  • Regulations
  • Small Molecules

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech