Antibody-NKG2D Ligand (Rae-1Beta) Fusion Protein for Breast Cancer Therapy

Abstract

Antibody-NKG2D ligand fusion protein targeted against the breast tumor antigen HER2. Using murine breast tumors designed to express the HER2 target antigen and the murine NKG2D ligand Rae-1BETA, we constructed an anti-HER2 IgG3-CH3-Rae-1BETA fusion protein by genetically fusing Rae-1BETA at the 3' end of CH3 domain of anti-HER2 IgG3 antibody. The purified Rae-I_ fusion proteins demonstrated ability to bind antigen on tumor cells and NKG2D on NK cells. The antibody-Rae-1_ fusion proteins showed increased cytotoxic activity directed against tumor targets.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2005
Accession Number
ADA446435

Entities

People

  • Seung-uon Shin

Organizations

  • University of Miami

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Antigens
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Department Of Defense
  • Governments
  • Intellectual Property
  • Lymphocytes
  • Molecules
  • Neoplasms
  • Patent Applications
  • Patents
  • Proteins
  • Tumor Cell Line
  • United States

Fields of Study

  • Biology
  • Medicine

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Immunology
  • Sensor Fusion and Tracking Systems.

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech