A Tissue Engineering Approach to Study the Progression of Breast Tumor Metastasis in Bone
Abstract
Most patients dying of breast cancer suffer painful bone metastasis. It is our hypothesis that the invasive growth and progression of breast metastatic lesions in bone requires the participation of various constituents from "soil". A reconstitution of such "soil" for the growth of breast metastatic cells will provide tremendous insights into factors critical for breast cancer growth in bone. We will firstly use our basic calcium minerals to reconstitute the mineral part of bone environment and then study the interaction of breast cancer cells with bone minerals. Then we will culture osteoblasts or bone marrow stromal cells on calcium phosphate scaffolds and then study the growth of breast cancer cells in this engineered bone microenvironment. Finally we will xenograft the calcium phosphate scaffolds, filled with cultured breast cancer cells, into athymic mice and study the resultant tumor growth and progression in vivo. The defined approach proposed will enable us to evaluate and define each individual components of bone for their role in the progression of breast bone
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2005
- Accession Number
- ADA446566
Entities
People
- Daotai Nie
- Mingxin Che
Organizations
- Wayne State University