Cytolytic T Lymphocytes in Organotypic Breast Carcinoma Culture
Abstract
In the proposed studies, novel T cell immunotherapies against breast cancer will be developed based on studies demonstrating a positive correlation between T lymphocytic infiltration of these tumors and favorable clinical outcome. The major goal of the proposed studies is to isolate and characterize cytolytic T lymphocytes (CTL) with in vivo-like T cell receptors. The CTL provide the basis for adoptive CTL immunotherapy and active immunotherapy with CTtderived peptides/antigens. During the past 12 months of the study 14 breast carcinoma tissues were cultured in organotypic cultures (reconstructs) and mixed lymphocyte/tumor cultures (MLTC). T cell lines were established from 11 breast cancer specimens. One long-term breast cancer cell line and 7 fibroblast cell lines also were established. Cytolytic activity was demonstrated in 9 of 10 T cell lines tested. Two of 9 tested T cell lines were shown to have helper function.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2005
- Accession Number
- ADA446739
Entities
People
- Dorothee Herlyn
Organizations
- University of Pennsylvania