Role of Tumor Stroma in Prostate Carcinogenesis
Abstract
This project ultimately aims to identify the role of tumor stroma in inducing prostate carcinogenesis. The hypothesis to be tested is that tumor stroma, in the form of carcinoma-associated fibroblasts (CAFs) isolated from human prostate cancer patienttissues, can initiate malignant transformation of human embryonic stem cells (hESCs). In the past year, we have sper a good deal of time refining the protocols and techniques that allow these experiments to be completed with high reproducibility and confidence that the fibroblastic cells being tested are of a malignant pathology. Using these refined techniques, we have generated a number of tissue recombinants using CAFs plus hESCs. We have also generated a numbe of positive and negative control recombinations. These grafts will be harvested in early April 2006. Overall, this project is progressing well, with some major technical hurdles overcome. Whatever the outcome of these experiments is, we will contribute significantly to the current understanding of the role of tumor stroma in the process of prostate carcinogenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2006
- Accession Number
- ADA446857
Entities
People
- Renea A Taylor
Organizations
- Monash University