Proteomic Analyses of Nipple Fluid for Early Detection of Breast Cancer
Abstract
Our purpose is to combine the well-established method of breast nipple fluid collection with new proteomics methodology for analyses of complex protein mixtures, in order to seek a better test for early breast cancer. The scope of our work is far- reaching as our results could have a significant impact on the ability to detect occult breast cancers at earlier stages than is possible with current cancer detection methods. To date, we have completed a pilot test of 12 patients to optimize collection and lab storage and analysis conditions; collected 84 nipple aspirate fluid samples from controls; and collected 9 nipple aspirate fluid samples from cases. We have also modified the nipple aspirate fluid (NAF) collection protocol to collect NAF directly with capillary tubes. This new collection procedure is more efficient, providing more useable samples for lab analysis. We have run test samples with the SELDI-TOF methodology. Significant numbers of peaks were detected in those samples on lMAC4O chips thereby validating both the collection and analysis protocols. In summary, we have developed a workable protocol for nipple aspirate collection that produces consistent quantitative protein analysis. In the next year, we will continue sample collection and analyses.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2005
- Accession Number
- ADA446882
Entities
People
- Alexander Miron
- Frederick P. Li
- Judy Garber
Organizations
- Dana–Farber Cancer Institute