Diversity, Replication, Pathogenicity and Cell Biology of Crimean Congo Hemorrhagic Fever Virus

Abstract

This research project is a result of a collaboration between three research groups aimed at elucidating basic replication processes of CCHFV with the expected outcome of providing basic research reagents and establishing the foundation of knowledge necessary for discovery of vaccines and antiviral therapeutics for Crimean Congo hemorrhagic fever. Our major findings during the first year of support are the following: We have sequenced and cloned in expression vectors the L G and N viral proteins of CCHFV, strain 10200; we have cloned and expressed a mini-replicon RNA of CCHFV; we have expressed the N protein of CCHFV in bacteria and found RNA-binding activity; we have determined the processing and subcellular localization of the glycoproteins of CCHFV; we have sequenced the M gene of 5 divergent CCHFV isolates and found broadly reactive as well as group-specific neutralizing epitopes. Our results provide novel insights on the molecular biology of this understudied highly pathogenic human virus.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2005
Accession Number
ADA446914

Entities

People

  • Adolfo García-Sastre

Organizations

  • Icahn School of Medicine at Mount Sinai

Tags

DTIC Thesaurus Topics

  • Biomedical And Dental Materials
  • Bunyaviridae Infections
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Gene Expression
  • Genetics
  • Golgi Apparatus
  • Health Services
  • Medical Personnel
  • Microbiology
  • Polymeric Films
  • Proteins
  • Sequence Analysis
  • Viral Structures
  • Viruses

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Systems Analysis and Design
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology