Differential Mechanisms of Androgen Resistance

Abstract

We proposed to study the mechanisms of androgen resistance by focusing on androgen receptor mutations that arise due to the selective pressures of antiandrogen treatment. In vitro we examined the mechanistic actions of bicalutamide and flutamide in transfection studies in a variety of cell lines and on different androgen responsive promoters. We have shown that flutamide exhibits agonist action in a cell specific manner. In vivo we utilized two models to test our hypothesis, xenograft models of human prostate cancer cell lines and a novel transgenic mouse model that expresses a humanized androgen receptor in the TRAMP background. We are currently assessing tumor progression in both models and sequencing AR cDNAs from tumors with different endocrine status.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2005
Accession Number
ADA446991

Entities

People

  • Orla A. O'mahony

Organizations

  • University of Michigan

Tags

DTIC Thesaurus Topics

  • Androgen Receptors
  • Androgens
  • Biomedical Research
  • Cancer
  • Cell Line
  • Cells
  • Disease Attributes
  • Diseases And Disorders
  • Genetic Variation
  • Hormones
  • Mutations
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Resistance
  • Transfection
  • Xenografts

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Prostate Cancer Biology.