Early Detection of Prostate Cancer
Abstract
Acoustic wave sensors have been widely used for detection of various chemical and biological species in liquid media. An improved binding of Protein A and IgG molecules on QCM biosensors by modifying the gold surface of the quartz crystal with a 35nm polystyrene film followed by an acidic treatment was accomplished. The frequency shifts due to the binding of the Protein A and IgG were 220 Hz and 282 Hz respectively for direct binding onto the chip. There was an appreciable increase in the frequency shift when the polystyrene film was used as an interfacial layer. The shift with the polystyrene film for Protein A was 364 Hz (an increase of 65%) and for the IgG it was 391 Hz (an increase of 40%). Complementary Atomic Force Microscopy (AFM) studies were carried out to understand the parameters responsible for such improved biomolecular binding. The CNT novel nanostructure material was used as chemical interface to enhance the sensitivity of the acoustic wave sensors which resulted in five fold increase in the frequency shift. Immobilization of the fPSA at different concentrations on gold surface was achieved and corresponding sensor response were registered.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2005
- Accession Number
- ADA447674
Entities
People
- Bruce E. Bejcek
- Massood Atashbar
Organizations
- Western Michigan University