Identification of a Gene on Chromosome 18q21 Involved in Suppressing Metastatic Prostate Cancer

Abstract

In metastatic prostate cancer there is increased frequency of loss of genetic material from chromosome 18q. We previously identified two regions on chromosome 18q as the potential sites of tumor suppressor genes involved in metastasis suppression. To identify these genes, we created custom bacterial artificial chromosome (BAC) microarrays containing the genomic sequence at 18q21-18q23. Analysis of 34 prostate tumors using array comparative genomic hybridization revealed gains and losses within this region. Transfection of the metastatic prostate cancer cell line PC-3 with BAC clones containing the maspin and NET01 genes results in altered in vitro growth properties. In vivo analysis of the metastatic potential of PC-3 cells transfected with maspin reveals that maspin is involved in the seeding of tumor cells to bone. Additionally, we have evidence that increased copy number of the cadherin-7 gene plays a role in prostate cancer tumorigenesis.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2005
Accession Number
ADA447814

Entities

People

  • Teresa R. Johnson-pais

Organizations

  • University of Texas Health Science Center at San Antonio

Tags

DTIC Thesaurus Topics

  • Bone Diseases
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chromosomes
  • Cultured Cells
  • Epithelial Cells
  • Gene Expression
  • Genetics
  • Health Services
  • Medical Personnel
  • Neoplasms
  • Prostate Cancer
  • Tissues

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular and genetic basis of cancer.
  • Prostate Cancer Biology.

Technology Areas

  • Biotechnology