Prostasin's Role in Prostate Cancer

Abstract

We have shown, in an alternative model system (of the mouse bladder) via collaborative research, that prostasin serine protease is capable of attenuating LPS-induced inflammatory gene expression response, specifically the mRNA expression of inducible nitric oxide synthase (iNOS), by potentially intercepting cytokine signaling at the cytokine receptor level. A new candidate protein, gp13O, the signal transducer of the interleukin-6 receptor complex, has been proposed to be the 12O-13O-kDa tyrosinephosphorylated protein regulated by prostasin in the DU-145 and PC-3 cells, as presented in the original proposal. Efforts are underway to demonstrate the applicability of this new molecular mechanism in the prostate cancer cell line PC-3, which had been shown by others to respond to inflammatory challenges such as LPS and cytokines through upregulation of the iNOS gene expression, while over-expressing the gp13O signal transducer. This new working hypothesis directly related to the original proposal will potentially lead to better understanding of the role of inflammation in prostate cancer biology.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2005
Accession Number
ADA447815

Entities

People

  • Karl Chai
  • Limei Chen

Organizations

  • University of Central Florida

Tags

DTIC Thesaurus Topics

  • Cell Line
  • Cell Membrane Structures
  • Cell Movement
  • Cell Physiological Processes
  • Cells
  • Cytokines
  • Gene Expression
  • Inflammation
  • Molecules
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Proteins
  • Tissues

Fields of Study

  • Medicine

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Immunology and Pathology
  • Prostate Cancer Biology.