In Vivo Imaging of mdrla Gene Expression
Abstract
The authors' experience studying the MDR1 gene prompted them to initiate work on a novel animal model to study MDR1/mdr1 gene expression under a variety of normal and breast cancer-related physiological conditions. With the advent of new bioimaging technology and the advancement of efficient gene targeting strategies, they found an opportunity to apply these state-of-the-art molecular tools to their problem. The work performed with the support of this grant has enabled them to do the following: (1) engineer a targeting vector to allow insertion of a reporter (luciferase or HSV-tk) into the genomic locus of the mouse mdr1a gene; (2) create mouse embryonic stem cells in which a gene replacement/knock-in strategy was used to insert luciferase into the mouse mdr1a genomic locus; (3) demonstrate that luciferase expression in these cells requires Cre recombinase to bring luciferase in-frame with the translational start site of the mdr1a gene product; (4) show that the recombined configuration of mdr1/LUC, in its cDNA form, encodes a functional protein with luciferase activity; and (5) create both founder and Cre-recombinase expressing mouse strains for use in in vivo imaging experiments. Work performed to date has proved the feasibility of this approach. However, further refinements to the model are required.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2005
- Accession Number
- ADA447839
Entities
People
- Timothy W. Synold