Preliminary Studies Examining Near Ultraviolet Fluorescence and Raman Spectroscopy for Tissue Interrogation of Shock
Abstract
Non-invasive methods for the measurement of tissue oxygen saturation have long been sought for the detection of impending shock and the adequacy of resuscitation. Current methods such as those that require insertion of a catheter into the pulmonary artery or superior vena cava have attendant risks to the patient. Gastric tonometry, though considered to be minimally invasive, still requires insertion of a nasogastric tube into the stomach. We have found that we can obtain resonance Raman and fluorescence signals from the sublingual surface of the tongue in laboratory animals in a non-invasive manner, that correlate with lactate and oxygenation measurements of blood withdrawn by central venous catheterization. The resonance Raman measurements monitor hemoglobin oxygenation. The fluorescence measurements monitor NADH levels. In addition to being noninvasive the measurements are selective against contaminating signals from myoglobin in deep tissue. Even with low powered laser excitation the resonance Raman signals are quite strong, and both methods are adaptable for use with portable fiber optic components.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 31, 2005
- Accession Number
- ADA447979
Entities
People
- James Terner
Organizations
- Virginia Commonwealth University