Rapid Identification of Key Pathogens in Wound Infection by Molecular Means

Abstract

The purpose of this study is to develop a real-time PCR-based high throughput detection and identification method for important wound pathogens. This will enable us to provide accurate identification a great deal sooner than has been possible in the past. This is likely to result in improved clinical outcome, less chance of bacterial resistance, and cost savings. To date, we have developed over 20 group-or species-specific primer/probe sets for pathogens encountered in wound infection. The specificities of the primer/probe sets were successfully evaluated with a battery of bacteria. The evaluation of sensitivity is underway. When the entire set of primers and probes has been completed, we will be able to detect, identify and semi-quantitate the important wound pathogens within 8 hours using this TaqMan PCR assay.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2006
Accession Number
ADA448241

Entities

People

  • Robert Bennion
  • Sydney Finegold

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Bacteria
  • Bacteriology
  • Bacteroidaceae
  • Biomedical Research
  • Detection
  • Health Services
  • Infection
  • Medical Personnel
  • Microbiology
  • Microorganisms
  • Pathogenic Bacteria
  • Wound Infections
  • Wounds And Injuries

Readers

  • Molecular Genetics
  • Trauma Surgery or Emergency Medicine.