Temporal and Spatial Dynamics of DNA Topoisomerase I in Prostate Cancer
Abstract
The purpose of this proposal was to investigate the response of prostate cancer to a series of camptothecin analogs, which are drugs that target DNA topoisomerase I. This final report summarizes the accomplishments toward the tasks outlined in the original proposal for this grant. The goal of the project was to try to understand why, despite the availability of topoisomerase I in prostate cancer, chemotherapeutic agents that target this enzyme are ineffective in treating prostate cancer. Members of the camptothecin family that specifically target topo I were studied in prostate cancer cell lines grown in vitro. The project was meant to attack this problem from three separate directions using three assays. The first assay was that of initial topo I distribution in normal and cancer cells and its response to camptothecin analog treatment. The second assay was for selection of camptothecin analogs that form slowly reversing topo I-DNA complexes. The third was measurement of the onset of apoptosis in prostate cells treated with the different analogs. This final report documents the work performed throughout the lifetime of the funded project. The authors note that while some experimental problems were encountered that limited the completion of all tasks, good progress was made overall on the proposed research.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2006
- Accession Number
- ADA448431
Entities
People
- Randy M. Wadkins
Organizations
- University of Mississippi