Enhancement of Skeletal Muscle Repair by the Urokinase-Type Plasminogen Activator System
Abstract
Skeletal muscle injuries, caused by intense exercise or trauma, are among the most common injuries in military personnel. Enhancement of muscle repair following injury would minimize time lost and maximize performance during training and combat. We and others have published data demonstrating that the extracellular protease urokinase-type plasminogen activator (uPA) is required for efficient muscle repair, although the underlying mechanisms remain to be elucidated. In the present project, immunofluorescence analysis demonstrated that cell proliferation is accelerated in injured muscle of PAl-1 null mice compared to wild-type mice. Immunofluorescence analysis also demonstrated that satellite cell accumulation is increased in injured muscle of PAl-1 null mice compared to wild-type mice. Western blot analysis supports accelerated cell proliferation and satellite cell accumulation in injured muscle of PAl-1 null mice. Taken together, these data indicate that satellite cell activity is enhanced in injured muscle of PAl-1 null mice. Findings from continued work on this project will provide insight into potential manipulation of components of the plasminogen system as a way to enhance muscle repair. Enhancing muscle repair following injury would minimize time lost due to muscle injury both during training and combat, and maximize performance following return from injury.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2006
- Accession Number
- ADA448526
Entities
People
- Timothy J Koh
Organizations
- University of Illinois at Chicago