Producing a Mouse Model to Explore the Linkages Between Tocopherol Biology and Prostate Cancer
Abstract
A mouse model of prostate cancer i.e. male C57/BL6 mice hemizygous for TRAMP and hetero or homozygous for tocopherol(TOC) transport protein (TTP) knockout was produced. C57/bl6 mice carrying the SV40 T antigen driven by the rat probasin promoter construct (TRAMP+) were bred with TTP knockout C57/bl6 mice, offspring with the desired sex and genotypes were raised on normal mouse chow. Animals were killed at multiple ages and GI tract, prostate pathology and plasma TOC contents were assessed. The results showed no difference in tumor development between the different TTP genotypes and their associated altered plasma levels of aT and gT. These strongly suggested that a reduction of plasma (and presumptively tissue, though unmeasured in this study) tocopherol achieved by manipulating the levels of TTP does not accelerate the course of prostate tumor formation. This raises questions regarding the possible effectiveness of tocopherol supplementation as treatment strategy for prostate cancer with possible next steps using direct supplemental feeding studies of both different tocopherols as well as these tocopherols metabolites in a TRAMP model to assess their roles in the development and progression of TRAMP induced prostate tumors.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2005
- Accession Number
- ADA448576
Entities
People
- Paul H Davis
Organizations
- University of California