Assessment of Neutralising Activity of Colostrum-Derived, Polyclonal, Bovine Antibodies: Use of the J774A.1 Anthrax Lethal Toxin Cytototoxity Assay

Abstract

Bacillus anthracis lethal toxin is believed to play an important role in anthrax pathogenesis. Published reports have suggested that antibody therapies with specificity against lethal toxin may improve survival in animal models. Macrophage cell lines such as J774A.1 provide fast, convenient models to measure LeTx activity. Here, we report the use of the J774A.1 cytotoxicity assay to assess the neutralising activity of colostrum-derived, polyclonal, bovine antibodies. Antibodies against lethal factor and protective antigen were found to protect macrophages from lethal toxin, and should be investigated further as a treatment for anthrax.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2005
Accession Number
ADA449511

Entities

People

  • Alison K. Pickering
  • Malcolm R. Alderton

Organizations

  • Defence Science and Technology Group

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Animals
  • Australia
  • Biological Warfare
  • Blood
  • Bodily Secretions
  • Cell Line
  • Cells
  • Culture Techniques
  • Infection
  • Macrophages
  • Proteins
  • Rodents
  • United States
  • Universities
  • Vaccines
  • Warfare
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Computational Modeling and Simulation
  • Immunology
  • Microbial Pathology