Serum Profiling for the Discovery of Biomarkers Associated With Low Level Vapor Exposure to VX GB in the Rat and Minipig Model
Abstract
The need to develop a rapid, low-level chemical nerve agent exposure detection system necessitates the development of a blood test that does not require individual initial baseline measurements. Toward this goal, we have begun a series of experiments to screen serum from animals exposed to low levels of OP nerve agents for biomarkers associated with exposure. This manuscript describes the use of strong anion exchange chromatography and Surface Enhanced Laser Desorbtion Ionization (SELDI)-Mass Spectrometry (MS) to efficiently screen serum for protein biomarkers of exposure to GB and VX in the minipig and rat models, respectively. Our most recent data indicates that several protein species are uniquely altered in the serum of low-level agent-exposed animals up to one week after exposure.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 16, 2004
- Accession Number
- ADA449522
Entities
People
- B. J. Benton
- C. L. Crouse
- D. B. Miller
- J. A. Scotto
- J. Manthei
- J. S. Forster
- K. L. Matson
- M. C. Orehek
- S. W. Hulet
- T. Muse
Organizations
- Edgewood Chemical Biological Center