Detection of Toxins Using Immobilized Carbohydrates as Recognition Elements
Abstract
Carbohydrate-protein interactions are important in many recognition processes that occur on cell surfaces. Bacteria, viruses and toxins use these interactions to bind to the host cells and confer pathogenic properties. These include botulinum, cholera, tetanus, diphtheria, and shiga-like (verotoxins) toxins, as well as influenza virus. In this study, we employed immobilized carbohydrate receptors on an array biosensor to detect protein toxins. Two carbohydrate derivatives: N-acetylneuraminic acid, also known as sialic acid (Neu-5Ac), and N-acetylgalactosamine (GalNAc) were immobilized on planar waveguides and interrogated with fluorescently labeled cholera and tetanus toxins. Signals obtained were dose-dependent and indicated that the surface density of the immobilized sugars affected toxin binding.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 16, 2004
- Accession Number
- ADA449596
Entities
People
- Chris R. Taitt
- Frances Ligler
- Miriam M. Ngundi
Organizations
- United States Naval Research Laboratory