BioSim (trademark) BG Non-Biological Aerosol Simulant

Abstract

A non-biological simulants has been developed for use as a safe effective alternative to Bacillus globigii (Bg). The simulant consists of one-micron polystyrene carrier beads with an aerodynamic diameter similar to anthrax spores, with total genomic Bg DNA attached to the surface. The DNA attachment to the bead is robust enough to withstand aerosolization and collection processes, yet reacts in a PCR-based detector using existing analytical protocols. BioSim(TradeMark) Bg can be used for hazard characterization, agent fate studies, detection equipment verification, training, and as an operator confidence check. The patent-pending delivery system allows the user to easily add a known dose of the simulant directly into a chamber, sampler inlet, or surface. Subsequent analysis with a PCR-based detector provides immediate operator feedback confirming that equipment and procedures are working properly.

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Document Details

Document Type
Technical Report
Publication Date
Nov 17, 2004
Accession Number
ADA449623

Entities

People

  • Andrew E. Page
  • David S. Alburty
  • Freeman Swank
  • Jennifer L. Dannehl
  • Kelly L. Brown
  • Richard R. Thomas

Organizations

  • MRIGlobal

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acceptance Tests
  • Aerosols
  • Biological Aerosols
  • Biological Detection
  • Boiling Point
  • Chemical Reactions
  • Detection
  • Detectors
  • Dielectric Polymers
  • Environment
  • Materials
  • Mixtures
  • Particles
  • Polymerase Chain Reaction
  • Propellants
  • Test And Evaluation
  • Vapor Pressure

Fields of Study

  • Physics

Readers

  • Aerosol Science/Aerosol Physics
  • Molecular Genetics
  • Software Engineering