A Method to Determine British Anti-Lewisite in Plasma Utilizing Gas Chromatography/Mass Spectrometry

Abstract

British anti-Lewisite (BAL) (2,3-dimercapto-1-propanol) is a potential therapeutic compound against the effects of cutaneous sulfur mustard, and an method for its determination in plasma has been developed. BAL and the internal standard (IS) ethane dithiol were removed from plasma samples through solid phase extraction, then reacted with 1-pentafluoropropionylimidazole (PFPI), forming stable pentafluoropropionyl derivates that are sensitive to gas chromatography/mass spectroscopy (GC/MS) analysis. Examining concentrations versus peak area ratios of BAL and IS derivatives demonstrated the method to be linear from 0.48 to 124 ng/ml in plasma with acceptable accuracy and precision. This method has proven to be both a reliable and sensitive means to determine BAL in plasma.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2005
Accession Number
ADA449696

Entities

People

  • Bebedict R. Capacio
  • Christopher E. Byers
  • Evan R. Holloway
  • John R. Smith
  • William D. Korte

Organizations

  • United States Army Medical Research Institute of Chemical Defense

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Accuracy
  • Alcohols
  • Blood Proteins
  • Chlorides
  • Detection
  • Electrons
  • Elements
  • Heavy Metals
  • Ions
  • Mass Spectrometry
  • Precision
  • Proteins
  • Reaction Time
  • Regression Analysis
  • Rodents
  • Standards

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry