Application of Sirna Technology to Manipulate Factors Involved in Acetylcholine Exocytosis and Botulinum Toxicity

Abstract

We demonstrated that the RhoB signaling pathway, regulates ACh release via actin cytoskeletal reorganization and that botulinum toxin type A (BoNT) inhibits neuroexocytosis by targeting the RhoB pathway in nerve growth factor-differentiated PC12 cells. To confirm these facts, small interfering RNA (siRNA) was used to knockout the expression of RhoB. Transfection of PC12 cells by the siRNA resulted in about 70% reduction of both mRNA and RhoB expression. This siRNA-induced RhoB suppression totally inhibited ACh release actin reorganization. The results of these studies strongly suggest that RhoB is involved in ACh exocytosis, likely that RhoB is a target of BoNT.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2005
Accession Number
ADA449700

Entities

People

  • Hiroshi Ishida
  • Kelly Erickson
  • Prabhati Ray

Organizations

  • Walter Reed Army Institute of Research

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Antibodies
  • Biology
  • Biotechnology
  • Cell Membrane
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Techniques
  • Cytoskeleton
  • Growth Factors
  • Materials
  • Membranes
  • Molecular Biology
  • Proteins
  • Synapses
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular and Cellular Biochemistry