Detoxification of Chemical Warfare Agents by the Plant Cholinergic System

Abstract

Plants have cholinesterases (pChEs), anti-ChEs, and activators of ChEs. We have isolated pChE from mung bean sprout. We investigated 300 plants and found that 75% of them contained anti- ChE that inhibited ChEs. Thirty-five percent contained activators of ChEs. An activator of ChE from wheat leaf, Tritiacche-T123 , activates fetal bovine serum AChE, equine BChE, and pChE. This non-oxime natural plant product may offer a new approach to the reactivation of OP-inhibited ChEs. pChE showed substrate differences from human acetylcholinesterase (AChE) or butyrylcholinesterase, hydrolyzing propionylthiocholine > acetylthiocholine > butyrylthiocholine. pChEs might also be useful as bioscavengers of OP nerve agents.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2005
Accession Number
ADA449752

Entities

People

  • Bhupendra P. Doctor
  • Deborah Moorad-doctor
  • Gregory E. Garcia
  • Haim N. Leader
  • Rajendra Gupta
  • Richard K. Gordon
  • Suman S. Thakur

Organizations

  • Walter Reed Army Institute of Research

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Acetylcholinesterases
  • Alcohols
  • Chemical Compounds
  • Chemical Warfare
  • Chemical Warfare Agents
  • Column Chromatography
  • Enzyme Inhibitors
  • Enzymes
  • Gel Electrophoresis
  • Gels
  • Nerve Agents
  • Organophosphates
  • Poisoning
  • Substrates
  • Warfare
  • Wheat

Fields of Study

  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Neurotoxicology