Detection of Biological Agents via a Novel Biopolymeric Assembly

Abstract

Nucleic acid-based biosensors can be very selective however it would be desirable to reduce the reagent load, remove unstable fluorescent labels and make the sensing element reusable. This may be achieved with electrochemical methods utilizing an electrode which is controlled by "molecular-scale gates" capable of being activated by a biological agent. In this project, chitosan covers the electrode and creates pathways for redox molecules to flow from the electrolyte to the electrode surface. Nucleic acid probes serve as switches, when unhybridized the pathways are open and will close upon the introduction of complementary nucleic acid sequence from the analyte.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2005
Accession Number
ADA449755

Entities

People

  • Gregory F Payne
  • Hyunmin Yi
  • James B. Gillespie
  • James J. Sumner
  • Li-qun Wu
  • William E. Bentley

Organizations

  • United States Army Research Laboratory

Tags

Communities of Interest

  • Biomedical
  • Energy and Power Technologies
  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Abstracts
  • Acids
  • Biological Factors
  • Biosensors
  • Detectors
  • Electrochemistry
  • Electrodeposition
  • Electrodes
  • Electronics
  • Elements
  • Hybridization
  • Molecular Electronics
  • Molecules
  • Monitoring
  • Nucleic Acids
  • Polysaccharides
  • Sequences

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry
  • Integrated Circuit Design and Technology.
  • Nanocomposite Materials Science

Technology Areas

  • Biotechnology