Development of Nucleic Acid Aptamer-Based Sensors for the Direct Detection and Identification of Biological Agents

Abstract

The objective of this work is to develop a novel sensing platform for the direct and rapid detection of biological agents. To achieve this goal, we are building on our portable DNA Biological Detection System work by incorporating a new class of molecular recognition elements, known as aptamers. In this paper, the nucleic acid-based sensor work conducted in our laboratory is discussed including initial work on the design and optimization of aptamer biorecognition sensor elements targeting a relevant biological agent surrogate (i.e., Bacillus globigii) and the development of a portable nucleic acid detection system.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2005
Accession Number
ADA449757

Entities

People

  • Dimitra N Stratis-Cullum
  • James B. Gillespie

Organizations

  • United States Army Research Laboratory

Tags

Communities of Interest

  • Sensors

DTIC Thesaurus Topics

  • Acids
  • Biological Factors
  • Chain Reactions
  • Chemical Reactions
  • Detection
  • Detectors
  • Electrophoresis
  • Fibers
  • Gel Electrophoresis
  • Identification
  • Laser Diodes
  • Lasers
  • Molecules
  • Nucleic Acids
  • Polymerase Chain Reaction
  • Polymers
  • Standards

Fields of Study

  • Chemistry

Readers

  • Molecular Genetics
  • Nanoscale Plasmonic Nanotechnology
  • Sensor Fusion and Tracking Systems.

Technology Areas

  • Biotechnology