Reversible Antibody Trap for Selective Sensor Devices

Abstract

PRELIMINARY RESULTS: (1) Achieved total reversibility of protein adsorption on pNIPAM films; (2) Can capture protein from very dilute solutions; (3) Adsorption behavior of antibodies on pNIPAM is simlar to other proteins, except there is a tendency to form multilayers; (4) Antibody adsorption is reversible; (5) Currently quantifying binding activity as a function of surface coating for a series of antibodies (native and engineered); (6) Designs for integration of heating, SAW devices are being developed. CONCLUSIONS: (1) Developing a small, reusable, highly selective sensor; (2) Can be programmed for just one antigen or a series of antigens by using a sensor array; (3) May be programmed on the spot for any antigens for which antibodies are available; (4) After use, the spent antibodies can be flushed, and the same or different antibodies can be adsorbed; (5) Can be integrated into existing Sandia Micro-chemlab platform.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2005
Accession Number
ADA449781

Entities

People

  • Bruce C. Bunker
  • Dale L. Huber
  • George C. Bachand
  • Ronald P. Manginell
  • Susan M. Brozik

Organizations

  • Sandia National Laboratories

Tags

DTIC Thesaurus Topics

  • Acoustic Properties
  • Adsorption
  • Advanced Materials
  • Antibodies
  • Chemistry
  • Detectors
  • Films
  • Homeland Defense
  • Hydrophilic Properties
  • Hydrophobic Properties
  • Materials
  • Materials Science
  • Phase Transformations
  • Proteins
  • Reversible
  • Security
  • Surface Acoustic Wave Devices

Readers

  • Immunology
  • Integrated Circuit Design and Technology.
  • Thermal Physics or Thermal Science.