DNA Cleavage Using Artificial Hydrolases Based on Cobalt (III) Cyclen Complexes

Abstract

We are developing small, robust catalytic systems to efficiently degrade DNA/RNA with a high degree of specificity. Here, we describe the use of tetra-azamacrocyle-chelated Co(III) complexes for the effective hydrolysis of double stranded plasmid DNA. These chelator molecules have been functionalized with a coupling group and we examine whether the modification has affected the ability of the complex to degrade DNA. Hydrolysis of DNA does appear to be slowed by the intermediate functionalization, but the eventual form of the coupled chelator-complex shows very high activity against a model pBluescript supercoiled DNA. We discuss these results, future tests, and plans for using these systems for sensitive equipment decontamination.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2005
Accession Number
ADA449797

Entities

People

  • D. A. Knight
  • Eddie Chang
  • Ellen R Goldman
  • Frederick Strich
  • James B. Delehanty
  • Jason Bongard
  • Rajeev Soni

Organizations

  • United States Naval Research Laboratory

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biomolecules
  • Catalysts
  • Chemical Compounds
  • Chemistry
  • Decontamination
  • Engineering
  • Enzymes
  • Hydrolases
  • Hydrolysis
  • Information Operations
  • Materials
  • Military Research
  • Nucleic Acids
  • Recognition
  • Sequences

Readers

  • Molecular Genetics
  • Molecular and Cellular Biochemistry
  • Organic Chemistry