Functional Analysis of Chk2-Kiaa0170 Interaction

Abstract

We characterized one mechanism how BRCA1, a downstream effector of MDC1 and Chk2, in maintaining genomic stability. We show that BRCA1 interacts with topoisomerase II and regulates topoisomerase II activity and localization. In addition, we characterized the interaction between MDC1 and DNA-PKcs/Ku, an upstream regulator of Chk2. We show that MDC1 regulates DNA-PKcs phosphorylation and localization, and participates in DNA repair. Finally, we generated MDC1-/-mice and in the process of evaluating the physiological function of MDC1. Our preliminary data suggest that MDC1 plays an important role in DNA damage response and maintaining genomic stability. In summery, our studies revealed novel mechanisms of the DNA damage response pathway and our animal model of the MDC1 knockout mice will elucidate how dysfunction of the Chk2-MDC1 pathway contributes to the development of tumor.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2005
Accession Number
ADA449840

Entities

People

  • Lou Zhenkun

Organizations

  • Mayo Clinic

Tags

DTIC Thesaurus Topics

  • Biology
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Chromosome Structures
  • Department Of Defense
  • Epithelial Cells
  • Functional Analysis
  • Genetics
  • Ionizing Radiation
  • Molecular Biology
  • Neoplasms
  • Proteins
  • Statistical Analysis
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Oncology (Cancer Research).
  • Prostate Cancer Biology.

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech