Activation of Mitogen-Activated Protein Kinases and Cyclic Amp Response Element-Binding Protein in Synaptic Plasticity

Abstract

Current evidence supports a critical role for cAMP in synaptic plasticity. Forskolin increases adenylyl cyclase activity to generate cAMP which induces a long-lasting potentiation of excitatory postsynaptic potentials in the hippocaznpal dentate gyrus to 137 plus or minus 5% of control, which persists for at least 60 min after forskolin removal. Blockade of NMDA (N-metbyl-D-aspartate) receptors with 20 micrometers 2-amino-5-phosphonovalerate (APV) or L-type calcium channels with 10 micrometers nifedipine, reduced this potentiation. TMB-8 (8- (diethylamino) octyl-3, 4, 5-trimethoxybenzoate, 50 micrometers), which interferes with calcium release from an inositol-3, 4, 5- trisphosphate (IP3) receptor-sensitive internal pool, also reduced forskolin potentiation. These data indicate that calcium from both extracellular and intracellular pools mediate the long-lasting,cAMP-mediated potentiation induced with forskolin in the dentate gyrus.

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Document Details

Document Type
Technical Report
Publication Date
Jul 11, 1997
Accession Number
ADA449874

Entities

People

  • Pamela J. Voulalas

Organizations

  • Uniformed Services University of the Health Sciences

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Alkenes
  • Amino Acids Peptides And Proteins
  • Biomolecules
  • Brain
  • Carrier Proteins
  • Chemical Compounds
  • Information Operations
  • Micrometers
  • Phosphoamino Acids
  • Plastic Properties
  • Proteins

Fields of Study

  • Biology

Readers

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  • Neuroscience
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