Role of CYP1B1 in PAH-DNA Adduct Formation and Breast Cancer Risk

Abstract

The cytochrome P450 enzyme CYP1B1 is a major enzyme involved in metabolizing polycyclic aromatic hydrocarbons (PAHs) to reactive intermediates. Given that exposure to carcinogenic PAHs may be a breast cancer risk factor, we have set out to investigate molecular mechanisms of the relationship between PAH exposure, CYP1B1 expression and breast cancer risk in a clinic-based case-control study. We have collected nontumor breast tissue from 43 women (32 cases and 11 controls) undergoing surgery and analyzed these specimens for CYP1B1 gene expression, CYP1B1 genotype and PAH-DNA adducts. CYP1B1 transcript levels determined by quantitative RT-PCR, varied more than 1000-fold between individuals. DNA adduct levels varied 10-fold between individuals. Because of the delayed start of the project, we have applied for and obtained a no cost extension until 10/27/07.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2006
Accession Number
ADA450453

Entities

People

  • Regine Goth-goldstein

Organizations

  • University of California, Berkeley

Tags

DTIC Thesaurus Topics

  • Aromatic Compounds
  • Aromatic Hydrocarbons
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cells
  • Cyclic Hydrocarbons
  • Cytochromes
  • Epithelial Cells
  • Ethnic Groups
  • Gene Expression
  • Genes
  • Genetic Variation
  • Genetics
  • Genotypes
  • Hydrocarbons
  • Neoplasms

Readers

  • Molecular Biology and Genetics
  • Organic Chemistry
  • Women's Health and Cancer Risk Research: African American Women and Pregnancy Outcomes.